pf 543 Search Results


compound 7 comp 7  (Echelon Biosciences)
92
Echelon Biosciences compound 7 comp 7
Compound 7 Comp 7, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf+543/pmc06278267__molecules___23___02750___s001-14-7-13?v=Echelon+Biosciences
Average 92 stars, based on 1 article reviews
compound 7 comp 7 - by Bioz Stars, 2026-08
92/100 stars
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pf-543  (MedChemExpress)
N/A
PF-543 Citrate (Sphingosine Kinase 1 Inhibitor II Citrate) is a potent, selective, reversible and sphingosine-competitive SPHK1 inhibitor with an IC50 of 2 nM and a Ki of 3.6 nM. PF-543 Citrate is >100-fold selectivity for
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pf 543  (Selleck Chemicals)
93
Selleck Chemicals pf 543
Pf 543, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf+543/pm39875359-181-2-14?v=Selleck+Chemicals
Average 93 stars, based on 1 article reviews
pf 543 - by Bioz Stars, 2026-08
93/100 stars
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sphingosine kinase 1 sphk1 inhibitor pf 543 hydrochloride  (Tocris)
89
Tocris sphingosine kinase 1 sphk1 inhibitor pf 543 hydrochloride
Inhibition of endogenous <t>SPHK1</t> has no effect on early inner ear development. (A) Morphological aspect of organotypic cultures after 20 h in serum-free medium (SFM), 1 μM PF-543, 10 nM IGF-1, IGF-1 plus PF-543. Immunostaining with TUNEL (cyan) and PH3 (red) of at least 5 otic vesicle per condition. Scale bar: 150 μm. (B) Quantification of otic vesicle area, (C) TUNEL-positive or (D) PH3-labeled cells in the presence or absence of PF-543 (0.25 and 1 μM) and IGF-1 (10 nM). (E) SOX2 intensity (F) and Tuj-1 intensity as neurogenesis markers normalized to data for SFM. Data are represented as mean ± SEM from at least n = 4 otic vesicles per condition. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s (B,E) or Dunnett’s T3 post hoc test (C) . * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.
Sphingosine Kinase 1 Sphk1 Inhibitor Pf 543 Hydrochloride, supplied by Tocris, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf+543/pmc08220815-48-38-45?v=Tocris
Average 89 stars, based on 1 article reviews
sphingosine kinase 1 sphk1 inhibitor pf 543 hydrochloride - by Bioz Stars, 2026-08
89/100 stars
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pf-543  (Merck KGaA)
90
Merck KGaA pf-543
Inhibition of endogenous <t>SPHK1</t> has no effect on early inner ear development. (A) Morphological aspect of organotypic cultures after 20 h in serum-free medium (SFM), 1 μM PF-543, 10 nM IGF-1, IGF-1 plus PF-543. Immunostaining with TUNEL (cyan) and PH3 (red) of at least 5 otic vesicle per condition. Scale bar: 150 μm. (B) Quantification of otic vesicle area, (C) TUNEL-positive or (D) PH3-labeled cells in the presence or absence of PF-543 (0.25 and 1 μM) and IGF-1 (10 nM). (E) SOX2 intensity (F) and Tuj-1 intensity as neurogenesis markers normalized to data for SFM. Data are represented as mean ± SEM from at least n = 4 otic vesicles per condition. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s (B,E) or Dunnett’s T3 post hoc test (C) . * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.
Pf 543, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf+543/pmc06265344-31-3-11?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
pf-543 - by Bioz Stars, 2026-08
90/100 stars
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pf 543  (Pfizer Inc)
86
Pfizer Inc pf 543
Inhibition of endogenous <t>SPHK1</t> has no effect on early inner ear development. (A) Morphological aspect of organotypic cultures after 20 h in serum-free medium (SFM), 1 μM PF-543, 10 nM IGF-1, IGF-1 plus PF-543. Immunostaining with TUNEL (cyan) and PH3 (red) of at least 5 otic vesicle per condition. Scale bar: 150 μm. (B) Quantification of otic vesicle area, (C) TUNEL-positive or (D) PH3-labeled cells in the presence or absence of PF-543 (0.25 and 1 μM) and IGF-1 (10 nM). (E) SOX2 intensity (F) and Tuj-1 intensity as neurogenesis markers normalized to data for SFM. Data are represented as mean ± SEM from at least n = 4 otic vesicles per condition. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s (B,E) or Dunnett’s T3 post hoc test (C) . * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.
Pf 543, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pf+543/pm41860929-54-0-8?v=Pfizer+Inc
Average 86 stars, based on 1 article reviews
pf 543 - by Bioz Stars, 2026-08
86/100 stars
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pf-543-d5 hydrochloride  (Biosynth Carbosynth)
N/A
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pf 543 hydrochloride  (Tocris)
N/A
PF 543 hydrochloride
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pf 543  (Cayman Chemical)
N/A
PF 543 is a potent inhibitor of sphingosine kinase 1 SPHK1 IC 2 nM that inhibits sphingosine 1 phosphate S1P binding to human recombinant SPHK1 K 3 6 nM It is selective for SPHK1 over
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pf 543 citrate  (Biosynth Carbosynth)
N/A
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pf-543 hydrochloride  (TargetMol)
N/A
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Image Search Results


Inhibition of endogenous SPHK1 has no effect on early inner ear development. (A) Morphological aspect of organotypic cultures after 20 h in serum-free medium (SFM), 1 μM PF-543, 10 nM IGF-1, IGF-1 plus PF-543. Immunostaining with TUNEL (cyan) and PH3 (red) of at least 5 otic vesicle per condition. Scale bar: 150 μm. (B) Quantification of otic vesicle area, (C) TUNEL-positive or (D) PH3-labeled cells in the presence or absence of PF-543 (0.25 and 1 μM) and IGF-1 (10 nM). (E) SOX2 intensity (F) and Tuj-1 intensity as neurogenesis markers normalized to data for SFM. Data are represented as mean ± SEM from at least n = 4 otic vesicles per condition. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s (B,E) or Dunnett’s T3 post hoc test (C) . * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Ceramide Kinase Inhibition Blocks IGF-1-Mediated Survival of Otic Neurosensory Progenitors by Impairing AKT Phosphorylation

doi: 10.3389/fcell.2021.678760

Figure Lengend Snippet: Inhibition of endogenous SPHK1 has no effect on early inner ear development. (A) Morphological aspect of organotypic cultures after 20 h in serum-free medium (SFM), 1 μM PF-543, 10 nM IGF-1, IGF-1 plus PF-543. Immunostaining with TUNEL (cyan) and PH3 (red) of at least 5 otic vesicle per condition. Scale bar: 150 μm. (B) Quantification of otic vesicle area, (C) TUNEL-positive or (D) PH3-labeled cells in the presence or absence of PF-543 (0.25 and 1 μM) and IGF-1 (10 nM). (E) SOX2 intensity (F) and Tuj-1 intensity as neurogenesis markers normalized to data for SFM. Data are represented as mean ± SEM from at least n = 4 otic vesicles per condition. Statistical significance was calculated by one-way ANOVA followed by Bonferroni’s (B,E) or Dunnett’s T3 post hoc test (C) . * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.

Article Snippet: Culture treatments included serum-free medium (SFM, control condition), 10 nM IGF-1 (recombinant IGF-I; Roche Molecular Biochemicals, Basel, Switzerland), the CERK inhibitor adamantane-1-carboxylic acid (2-benzoylamino-benzothiazol-6-yl)amide (NVP-231, hereafter called CKi) (Tocris Bioscience, Bristol, United Kingdom) prepared in DMSO, and the sphingosine kinase 1 (SPHK1) inhibitor PF-543 hydrochloride (Tocris Bioscience).

Techniques: Inhibition, Immunostaining, TUNEL Assay, Labeling, Control